Versions

Description

Portcullis stands for PORTable CULLing of Invalid Splice junctions from pre-aligned RNA-seq data. It is known that RNAseq mapping tools generate many invalid junction predictions, particularly in deep datasets with high coverage over splice sites. In order to address this, instead for creating a new RNAseq mapper, with a focus on SJ accuracy we created a tool that takes in a BAM file generated by an RNAseq mapper of the user's own choice (e.g. Tophat2, Gsnap, STAR2 or HISAT2) as input (i.e. it's portable). It then, analyses and quantifies all splice junctions in the file before, filtering (culling) those which are unlikely to be genuine. Portcullis output's junctions in a variety of formats making it suitable for downstream analysis (such as differential splicing analysis and gene modelling) without additional work.

Repository

https://github.com/maplesond/portcullis.git

Project Slug

portcullis

Last Built

1 year, 1 month ago passed

Maintainers

Home Page

https://github.com/maplesond/portcullis

Badge

Tags

mapping, filtering, rnaseq, junctions, splicing

Project Privacy Level

Public

Short URLs

portcullis.readthedocs.io
portcullis.rtfd.io

Default Version

latest

'latest' Version

master