Welcome to Molecular Biology Protocols’s documentation!

Contents:

• Constitution of Reagent
  • Antibiotics
  • Buffer or Regents
  • Media
  • SDS-PAGE
  • Protein purification
  • Ni-NTA affinity column
• Protocols for |E.coli|
  • Create chemically competent cells
  • Transforming chemically competent cells
  • Colony PCR with KAPA Taq
  • Single Colony Isolation
• Protocols for DNA
  • Agarose gel electrophoresis
  • PCR Amplification
  • Restriction Digest
  • DNA Ligation
  • Ethanol precipitation
  • Sequencing
  • Compose vector and insert DNA
• Protocols for Protein
  • Measurement of protein concentration
  • SDS Polyacrylamide gel electrophoresis (SDS-PAGE)
  • IPTG induction test
  • IPTG induction
  • Affinity chromatography with His-Bind Resins

Indices and tables

• Index
• Module Index
• Search Page